Products/Services Used | Details | Operation |
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Gene Synthesis> | … Human FP- encoding DNA was synthesized (GenScript) with the endo- genous signaling peptide and a C-terminal 6ÂHis tag (Table 1). The final constructs were generated by the insertion of TEV protease sites using site-directed mutagenesis … | Get A Quote |
The 54 kDa protein properdin, also known as factor P (FP), plays a major role in the complement system through the stabilization of the alternative pathway convertases. FP circulates in the blood as cyclic dimers, trimers and tetramers, and this heterogeneity challenges detailed structural insight into the mechanism of convertase stabilization by FP. Here, the generation of an intact FP monomer and a variant monomer with the third thrombospondin repeat liberated is described. Both FP monomers were excised from recombinant full-length FP containing internal cleavage sites for TEV protease. These FP monomers could be crystallized, and complete data sets extending to 2.8 Å resolution for the intac... More