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Proteins, Expression, Isolation and Analysis> | … Agents Chemother. doi:10.1128/AAC.01524-18 Copyright © 2019 American Society for Microbiology. All Rights Reserved. on January 30, 2019 by guest http://aac.asm.org/ Downloaded from Page 2. 2 … 44 45 46 on January 30, 2019 by guest http://aac.asm.org/ Downloaded from …The fosAKP and fosA3 genes (including mutants) were synthesized with the inclusion of a C-terminal His6 tag by GenScript (Piscataway, NJ, USA) and cloned into pET-22(b) for protein expression and purification, as described previously (9). | Get A Quote |
The spread of multidrug or extensively drug-resistant Gram-negative bacteria is a serious public health issue. There are too few new antibiotics in development to combat the threat of multidrug-resistant infections, and consequently the rate of increasing antibiotic resistance is outpacing the drug development process. This fundamentally threatens our ability to treat common infectious diseases. Fosfomycin (FOM) has an established track record of safety in humans and is highly active against , including multidrug-resistant strains. However, many other Gram-negative pathogens, including the "priority pathogens" and , are inherently resistant to FOM due to the chromosomal gene, which directs express... More