Products/Services Used | Details | Operation |
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Bacterial Expression System> | Recombinant rGel was expressed in E. coli as an N-terminal 6× His tag fusion from synthetic genes, as reported by Rosenblum et al., with amino acids 112–119 deleted and codons optimized for expression in E. coli (Genscript, Piscataway, NJ) in the pET28a backbone. | Get A Quote |
Delivery of toxins, such as the ricin A chain, Pseudomonas exotoxin, and gelonin, using antibodies has had some success in inducing specific toxicity in cancer treatments. However, these antibody-toxin conjugates, called immunotoxins, can be bulky, difficult to express, and may induce an immune response upon in vivo administration. We previously reported delivery of a recombinant variant of gelonin (rGel) by the full-length prostate-specific membrane antigen (PSMA) binding aptamer, A9, to potentially circumvent some of these problems. Here, we report a streamlined approach to generating aptamer-rGel conjugates utilizing a chemically synthesized minimized form of the A9 aptamer. Unlike the full-length A9 aptamer... More