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Bacterial Expression System> | The gene coding for the α-isoform of PP1 (residues 1–330) fused with an N-terminal TEV cleavable hexahistidine tag was synthesized by GenScript and subcloned into pMAL-c2G under control of the Ptac promoter. | Get A Quote |
Rif1 is a conserved protein that plays essential roles in orchestrating DNA replication timing, controlling nuclear architecture, telomere length and DNA repair. However, the relationship between these different roles, as well as the molecular basis of Rif1 function is still unclear. The association of Rif1 with insoluble nuclear lamina has thus far hampered exhaustive characterization of the associated protein complexes. We devised a protocol that overcomes this problem, and were thus able to discover a number of novel Rif1 interactors, involved in chromatin metabolism and phosphorylation. Among them, we focus here on PP1. Data from different systems have suggested that Rif1-PP1 interaction is conserved and ha... More