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Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling.

Allergy Asthma Immunol Res. 2015;

YinSui Chu，LiaoEn Chih，ChiuChih Liang，ChangChing Yun，TsaiJa

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Catalog Antibody	… Expression of soluble VHHs with an E-Tag was induced with IPTG (1 mM) The recombinant VHHs-E-Tag protein was extracted from the periplasm and tested for its capacity to recognize Nsp9 using an ELISA with an anti-E-Tag antibody (Genscript, Piscataway, NJ, USA) …	Get A Quote

摘要

House-dust-mite (HDM) major allergen Der p2 shares homology and function with Toll-like receptor (TLR) signaling protein myeloid differentiation-2 (MD2) and may lead to airway inflammation. Should Der p2 be internalized by human airway epithelium， it has the theoretical propensity to potentiate epithelium activation. This study aimed to demonstrate the internalization of Der p2 by airway epithelium and to investigate the effects of Der p2 on MD2 expression and epithelium activation.