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Proteins, Expression, Isolation and Analysis> | … Purified rDer p2-EGFP was further clarified by Endotoxin Detoxi-Gel (Thermo, Wilmington, DE, USA) to remove LPS Residual LPS was measured using the ToxinSensor Chromogenic LAL Endotoxin Assay kit (GenScript, Piscataway, NJ, USA) … | Get A Quote |
Localized surface plasmon resonance (LSPR) spectroscopy and imaging are emerging biosensor technologies which tout label-free biomolecule detection at the nanoscale and ease of integration with standard microscopy setups. The applicability of these techniques can be limited by the restrictions that surface-conjugated ligands must be both sufficiently small and orientated to meet analyte sensitivity requirements. We demonstrate that orientated single domain antibodies (sdAb) can optimize nanoplasmonic sensitivity by comparing three anti-ricin sdAb constructs to biotin-neutravidin, a model system for small and highly orientated ligand studies. LSPR imaging of electrostatically orientated sdAb exhibited a ricin ... More