| Products/Services Used | Details | Operation |
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| Codon Optimization | … In order to detect and purify the target proteins, they were equipped with a C-terminal hexahistidine tag The engineered genes were codon-optimized and synthesized by GenScript [Figure 1], Figure 1 Schematic overview of the synthesized rTvTS1-encoding gene … | Get A Quote |
Sialidases and trans-sialidases play important roles in the life cycles of various microorganisms. These enzymes can serve nutritional purposes， act as virulence factors or mediate cellular interactions (cell evasion and invasion). In the case of the protozoan parasite Trypanosoma vivax， trans-sialidase activity has been suggested to be involved in infection-associated anaemia， which is the major pathology in the disease nagana. The physiological role of trypanosomal trans-sialidases in host-parasite interaction as well as their structures remain obscure. Here， the production， purification and crystallization of a recombinant version of T. vivax trans-sialidase 1 (rTvTS1) are described. The obtained r... More
