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Gene Synthesis> | … the N-terminal 179 amino acids of PlyGVE2, the ΦGVE2 amidase endolysin [20] (NCBI accession number YP_001285830), and the C-terminal 53 amino acids from PlyCP26F (NCBI accession number YP_007004008), including a 6X His-tag [19], was synthesized by GenScript … | Get A Quote |
Clostridium perfringens is the third leading cause of human foodborne bacterial disease and is the presumptive etiologic agent of necrotic enteritis among chickens. Treatment of poultry with antibiotics is becoming less acceptable. Endolysin enzymes are potential replacements for antibiotics. Many enzymes are added to animal feed during production and are subjected to high-heat stress during feed processing. To produce a thermostabile endolysin for treating poultry, an E. coli codon-optimized gene was synthesized that fused the N-acetylmuramoyl-L-alanine amidase domain from the endolysin of the thermophilic bacteriophage ɸGVE2 to the cell-wall binding domain (CWB) from the endolysin of the C. perfringens-spe... More