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Mammalian Expression System> | … genes for the heavy- and light-chain variable and constant domains of 641 I-9 and the single chain Fv (scFv) of H2526 with a (GGGGS) 3 linker all with a noncleavable C-terminal His 6X tag, were synthesized and codon optimized for mammalian cell expression by GenScript … | Get A Quote |
Vaccines for rapidly evolving pathogens will confer lasting immunity if they elicit antibodies recognizing conserved epitopes, such as a receptor-binding site (RBS). From characteristics of an influenza-virus RBS-directed antibody, we devised a signature motif to search for similar antibodies. We identified, from three vaccinees, over 100 candidates encoded by 11 different VH genes. Crystal structures show that antibodies in this class engage the hemagglutinin RBS and mimic binding of the receptor, sialic acid, by supplying a critical dipeptide on their projecting, heavy-chain third complementarity determining region. They share contacts with conserved, receptor-binding residues but contact diff... More