| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … The lipid bilayer was exposed to the lysine‐containing peptide (168 μM in TBS, pH 72, Ac‐FKGGERCG‐NH 2 , Genscript, Piscataway, NJ, USA) for 50 min (10 μL min −1 ) Unreacted maleimides were quenched with β‐mercaptoethanol (5 mM; Sigma–Aldrich) for 5 min … | Get A Quote |
Antibody-like proteins selected from discovery platforms are preferentially functionalized by site-specific modification as this approach preserves the binding abilities and allows a side-by-side comparison of multiple conjugates. Here we present an enzymatic bioconjugation platform that targets the c-myc-tag peptide sequence (EQKLISEEDL) as a handle for the site-specific modification of antibody-like proteins. Microbial transglutaminase (MTGase) was exploited to form a stable isopeptide bond between the glutamine on the c-myc-tag and various primary-amine-functionalized substrates. We attached eight different functionalities to a c-myc-tagged antibody fragment and used these bioconjugates for downstream applic... More
