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Gene Synthesis> | … The novel protein is referred to as anti-GFP-nanobody-4K The designed sequence was synthesized as a BamHI/SalI fragment by GenScript (Piscataway, USA), and ligated into the same sites of pGEX-6P-1 (GE Healthcare, USA) … | Get A Quote |
With the recent development of single-molecule localization-based superresolution microscopy, the imaging of cellular structures at a resolution below the diffraction-limit of light has become a widespread technique. While single fluorescent molecules can be resolved in the nanometer range, the delivery of these molecules to the authentic structure in the cell via traditional antibody-mediated techniques can add substantial error due to the size of the antibodies. Accurate and quantitative labeling of cellular molecules has thus become one of the bottlenecks in the race for highest resolution of target structures. Here we illustrate in detail how to use small, high affinity nanobody binders against GFP an... More