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Custom Vector Construction> | … Methods Sample preparation A gene encoding residues 1–307 of the yeast ADP/ATP carrier 3 (yAAC3) with an N-terminal His 6 tag was synthesized by GenScript and was subsequently cloned into the pET21a expression vector … | Get A Quote |
The ADP/ATP carrier (AAC) transports ADP and ATP across the inner mitochondrial membrane. Unlike most transporters, which have two-fold direct or inverted quasisymmetry, AAC has apparent three-fold rotational symmetry. Further, its transport rate is relatively fast for transporters that carry large solutes. Here, we study the yeast AAC carrier 3 by obtaining comprehensive NMR relaxation dispersion measurements, which provide residue-specific information on the protein's conformational exchange. Our data indicate that AAC is predominantly in the cytosol-facing open state and converts to a sparsely populated state in an asymmetric manner despite its three-fold structural symmetry. Binding of the substra... More