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ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization.

Proc. Natl. Acad. Sci. U.S.A.. 2015;

SenguptaPrabuddha，Satpute-KrishnanPrasanna，SeoArnold Y，BurnetteDylan T，PattersonGeorge H，Lippincott-SchwartzJenn

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Plasmid DNA Preparation	The cDNAs for FKBP12 and the FRB domain of FRAP1 were synthesized by Genscript and provided in the plasmid pUC57. The FKBP12 cDNA was amplified from the plasmid pUC57-FKBP12 (from Genscript), using the N-terminal primer 5′-GATCGTCGACGATGGGAGTGCAGGTG-3′ containing a SalI site (underlined) and the C-terminal primer 5′-GATCGGATCCCGTTCCAGTTTTAGAAG-3′ containing a BamHI site (underlined). The FRB cDNA was amplified from the plasmid pUC57-FRAP1 (Genscript),	Get A Quote

摘要

Whether Golgi enzymes remain localized within the Golgi or constitutively cycle through the endoplasmic reticulum (ER) is unclear， yet is important for understanding Golgi dependence on the ER. Here， we demonstrate that the previously reported inefficient ER trapping of Golgi enzymes in a rapamycin-based assay results from an artifact involving an endogenous ER-localized 13-kD FK506 binding protein (FKBP13) competing with the FKBP12-tagged Golgi enzyme for binding to an FKBP-rapamycin binding domain (FRB)-tagged ER trap. When we express an FKBP12-tagged ER trap and FRB-tagged Golgi enzymes， conditions precluding such competition， the Golgi enzymes completely redistribute to the ER upon rapamycin treatme... More