Products/Services Used | Details | Operation |
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Biochemicals> | … China) Recombinant bovine enterokinase (rEK) with a His×6 tag at the C-terminus was purchased from GenScript Corporation (Shanghai, China) All other chemicals used were of reagent grade (Sigma, Guangzhou, China) 22 … | Get A Quote |
Insulin-like growth factor-II (IGF2) is a growth factor for the control of cell proliferation and apoptosis. To explore the clinical use of human IGF2, an efficient method for production of a large amount of active recombinant hIGF2 is necessary. Human IGF2 cDNA was cloned into pET32 vector where it is under the control of an IPTGinducible T7 promoter. High level soluble thioredoxin (Trx)-hIGF2 fusion protein was produced at room temperature following IPTG induction, amounting up to 20% of the total soluble bacterial proteins. The recombinant Trx-hIGF2 fusion protein was purified to an approximate 95% purity using Ni+-NTA affinity chromatography with an overall yield of 120 mg protein per liter of bacterial cul... More