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Custom Vector Construction> | … The two designed genes of OD1 (214aa, from 246 to 460 of gp120 1084i strain26, with a 6xHis-tag at the N- terminus) and OD2 (4 cysteine substitutions in OD1) were synthesized by GenScript, and were cloned into pET28b expression vector (Novagen) for protein expression … | Get A Quote |
The outer domain of gp120 is a relatively stable domain compared to the inner domain and bridging sheet at the CD4-binding site for the HIV-1 primary receptor. Therefore, the outer domain has been considered as an immunogen candidate for vaccine design. In this report, we focused on the VRC01 antibody binding epitope in the outer domain and evaluated the effects of introducing two disulfides to further stabilize the outer domain structure where the antibody binds for the purpose of generating a more effective immunogen. Our experimental data based on neutralization activities against HIV-1 of anti-sera produced from immunized guinea pigs demonstrated that this stabilized outer domain-based immunogen significant... More