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Immobilization of the recombinant (His) 6-tagged l-arabinose isomerase from Thermotoga maritima on epoxy and cupper-chelate epoxy supports

Food and bioproducts processing 2015. 2015;

Bortone, Nadia, Fidaleo, Marcello

Products/Services Used	Details	Operation
Plasmid DNA Preparation	… All reagents were of analytical grade 22 Plasmid and microorganism The expression vector was supplied by Genscript Corp (Piscataway, USA) Based on the published amino acid sequence of TMAI (Genbank accession no …	Get A Quote

摘要

l-Arabinose isomerase from the hyperthermophilic bacterium Thermotoga maritima (TMAI) was overexpressed in Escherichia coli as a (His)6-tagged protein and purified by heat treatment followed by immobilized Ni2+ affinity chromatography.