| Products/Services Used | Details | Operation |
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| Bacterial Expression System | … was cultured in LB medium at 16 °C. AbrB protein expression was induced at OD 600 0.6 with 0.3 mmol/L IPTG for 6 h. The AbrB protein with the His-tag was purified from E. coli cells using a Ni-nitrilotriacetic acid (NTA) purification system (GenScript Corporation, Piscataway, NJ … | Get A Quote |
The bacitracin synthetase gene cluster in Bacillus licheniformis DW2 is composed of the bacABC operon encoding a non-ribosomal peptide synthetase and bacT encoding a thioesterase. Although the bacitracin gene cluster has been well studied， little is known about how this gene cluster is regulated. This study provides insight into how the transcription factors Spo0A and AbrB regulate bacitracin biosynthesis. Deletion of spo0A resulted in drastically reduced expression of bacA and bacT， and subsequently bacitracin production. On the other hand， the expression of bacA and bacT increased significantly in B. licheniformis DW2ΔabrB and DW2Δ0AΔabrB compared to the wild-type strain DW2. The bacitracin yields on... More
