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Serum Samples From Middle-aged Adults Vaccinated Annually with Seasonal Influenza Vaccines Cross-neutralize Some Potential Pandemic Influenza Viruses.

J. Infect. Dis.. 2016;

WangWei，Alvarado-FacundoEsmeralda，ChenQiong，AndersonChristine M，ScottDorothy，VassellRussell，WeissCar

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Codon Optimization	… coding for the mature protein of the GG1 gene (GenBank: M16064, SwissProt ID: P084531) along with a C-terminal 6x Histidine tag and methionine as a start codon, which was codon optimized for expression in E coli, was obtained from GenScript (Piscataway Township, NJ …	Get A Quote

摘要

We examined serum samples from adults ages 48-64 who received multiple seasonal influenza vaccines from 2004 to 2009 for cross-neutralizing antibodies to potential pandemic strains. Using pseudoviruses bearing various hemagglutinins (HA-pseudoviruses)， we found serum neutralization titers (≥160) in 100% against A/Japan/305/1957 (H2N2)， 53% against A/Hong Kong/1073/99 (H9N2)， 56% against the H3N2 variant A/Indiana/08/11 (H3N2v)， 11% against A/Hong Kong/G9/97 (H9N2)， and 36% A/chicken/Hong Kong/SF4/01 (H6N1). None had titers >160 to A/Shanghai/2/13 (H7N9) or A/Netherlands/219/03 (H7N7). Thirty-six percent to 0% had neutralization titers to various H5N1 strains. Titers to H9， H6， and H5 HA-pseudovi... More