Products/Services Used | Details | Operation |
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Peptide Synthesis> | The membrane was incubated with a hybridization buffer containing an antiserum against His-tag peptide (GenScript, Piscataway, NJ, USA) during which the antiserum bound to His-tagged Cmr-2α protein. The His-tag antiserum was then recognized by a secondary antibody (Goat Anti-Mouse IgG, GenScript) and... | Get A Quote |
CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated) systems are widespread in archaea and bacteria, and research on their molecular mechanisms has led to the development of genome-editing techniques based on a few Type II systems. However, there has not been any report on harnessing a Type I or Type III system for genome editing. Here, a method was developed to repurpose both CRISPR-Cas systems for genetic manipulation in Sulfolobus islandicus, a thermophilic archaeon. A novel type of genome-editing plasmid (pGE) was constructed, carrying an artificial mini-CRISPR array and a donor DNA containing a non-target sequence. Transformation of a pGE plasmid would yield tw... More