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Monoclonal Antibody Services> | … The fractions were analyzed by 12% SDS-PAGE. Endotoxin of the purified rCPB2 proteins was removed using a Toxin Eraser TM Endotoxin Removal Kit (GenScript, USA) before use. 2.5. Generation of Hybridoma Cell Lines Producing Monoclonal Antibodies to CPB2 … | Get A Quote |
() beta2 toxin (CPB2) is an important virulent factor of necrotic enteritis in both animals and humans. However, studies of its pathogenic roles and functional mechanisms have been hampered due to the difficulty of purification and lack of specific antibodies against this toxin. A recombinant His-tagged beta2 (rCPB2) toxin and monoclonal antibodies (McAbs) against CPB2 were generated and characterized by assays of cytotoxicity, immunoblotting, ELISA, neutralization, and immunofluorescence. A His-tagged rCPB2 with integrity and cytotoxicity of native CPB2 was purified from expressing system, which exhibited a moderate cytotoxicity on NCM460 human intestinal epithelial cells. The rCPB2 could induc... More