Products/Services Used | Details | Operation |
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Peptide Synthesis> | Protein coding sequences for the large (HydA) and small (HydB) subunits with the signal peptide sequences (first 35 amino acids of HydB and last 25 amino acids of HydA) removed, and including a carboxy terminal StrepII tag sequence on HydA, were ordered from GenScript. | Get A Quote |
Hydrogenases catalyze the reduction of protons and oxidation of molecular hydrogen with high turnover frequencies and low overpotentials under ambient conditions. The heterodimeric [FeFe] hydrogenase from Desulfovibrio desulfuricans has an exceptionally high activity, and can be purified aerobically in an oxygen‐stable inactive state. Recently, it was demonstrated that monomeric [FeFe] hydrogenases produced recombinantly in Escherichia coli can be artificially maturated by simply incubating the inactive “apo” enzymes with the synthetic [2Fe] cofactor mimic [Fe2(adt)(CO)4(CN)2]2−. Here, we use the same technique to produce the heterodimeric “apo” hydrogenase from D. desulfuricans in E. coli with a hi... More