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Plasmid DNA Preparation> | … sequence of CarDHN, along with a 5′-untranslated sequence 5′-GTAACCAATTATTTCAGCA- 3′ from the tomato rbcS and a FLAG tag sequence 5′-GATTACAAGGATGATGATGATAAG- 3′ at the 3′ end, was synthesized and cloned in plasmid PUC57 by GenScript (USA) | Get A Quote |
Dehydrin proteins are known to serve a wide variety of intracellular protective functions and enhance the capability of plants to deal with abiotic stresses. The gene for a SK5 type dehydrin from the arctic chickweed plant Cerastium arcticum (CarDHN) was introduced into tobacco plants. Stress responses of the transgenic plants were evaluated. Compared to the untransformed wild type plants, the CarDHN-expressing plants exhibited improved tolerance to salt and osmotic stress during seed germination and seedling growth, and survived in −10 °C freezing treatment. These CarDHN plants also accumulated lower levels of free proline under salt stress and exhibited lower transcription levels of HSP70 and HSP26 under d... More