Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | he codon-optimized synthetic construct of the ZM176.66 HIV-1 subtype C gp120 containing aa 41–492 (HXB2 numbering) ΔV123 (core) was produced by GenScript with an N-terminal 6×-histidine tag and inserted into the pVRC- 8400 expression vector as described for Fabs. | Get A Quote |
Antibodies that neutralize autologous transmitted/founder (TF) HIV occur in most HIV-infected individuals and can evolve to neutralization breadth. Autologous neutralizing antibodies (nAbs) against neutralization-resistant (Tier-2) viruses are rarely induced by vaccination. Whereas broadly neutralizing antibody (bnAb)-HIV-Envelope structures have been defined, the structures of autologous nAbs have not. Here, we show that immunization with TF mutant Envs gp140 oligomers induced high-titer, V5-dependent plasma neutralization for a Tier-2 autologous TF evolved mutant virus. Structural analysis of autologous nAb DH427 revealed binding to V5, demonstrating the source of narrow nAb specificity and explaining... More