Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | Plasmid pJc1/5A-SNAP was generated by replacement of the GFP coding sequence (XbaI/RsrII) in pJc1/5A-GFP[K1402Q] with that of SNAPf cDNA (synthesized by GenScript). | Get A Quote |
Hepatitis C virus (HCV) NS5A is essential for viral genome replication within cytoplasmic replication complexes and virus assembly at the lipid droplet (LD) surface, although its definitive functions are poorly understood. We developed approaches to investigate NS5A dynamics during a productive infection. We report here that NS5A motility and efficient HCV RNA replication require the microtubule network and the cytoplasmic motor dynein and demonstrate that both motile and relatively static NS5A-positive foci are enriched with host factors VAP-A and Rab5A. Pulse-chase imaging revealed that newly synthesized NS5A foci are small and distinct from aged foci, while further studies using a unique dual fluorescent... More