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Proteins, Expression, Isolation and Analysis> | … and equalized for total protein concentration. Samples were subjected to SDS-PAGE (Genscript, Piscataway, NJ), followed by transfer to polyvinylidene difluoride membranes (Sigma). Membranes were probed with the following … | Get A Quote |
Desmoplakin (DP) is an obligate component of desmosomal cell-cell junctions that links the adhesion plaque to the cytoskeletal intermediate filament network. While a central role for DP in maintaining the structure and stability of the desmosome is well established, recent work has indicated that DP's functions may extend beyond cell-cell adhesion. In our study, we show that loss of DP results in a significant increase in cellular migration, as measured by scratch wound assays, Transwell migration assays, and invasion assays. Loss of DP causes dramatic changes in actin cytoskeleton morphology, including enhanced protrusiveness, and an increase in filopodia length and number. Interestingly, these... More