Products/Services Used | Details | Operation |
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Custom Vector Construction> | … from New England Biolabs. Ni-NTA His-trap column was provided from GE Healthcare Life Sciences. The recombin- ant plasmids were purchased from Genscript. Construction of recombinant plasmids The expression level … | Get A Quote |
Several protein expression systems can be used to get enzymes in required quantities and study their functions. Incorporating a polyhistidine tag is a beneficial way of getting various enzymes such as FDHs for industrial applications. The NAD dependent formate dehydrogenase from (CtFDH) can be utilized for interconversion of formate to carbon dioxide coupled with the conversion of NAD to NADH. In this study, N-terminal His tagged CtFDH (N-CtFDH) and C-terminal His tagged CtFDH (C-CtFDH) was constructed to learn the effect of His tag location on the activity and kinetic parameters of the enzyme. The solubility of proteins was not affected by tag position, however, an interference on the N-terminal region ... More