Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … Tag sequences were inserted into the viral genome by HR. Viral DNA was cotransfected along with a plasmid containing the tag sequences flanked by sequence homologies to the viral site of insertion (synthetically generated by GenScript, Piscataway, NJ, USA) … | Get A Quote |
Homologous recombination (HR) is considered a major driving force of evolution because it generates and expands genetic diversity. Evidence of HR between coinfecting herpesvirus DNA genomes can be found frequently both and in clinical isolates. Each herpes simplex virus type 1 (HSV-1) replication compartment (RC) derives from a single incoming genome and maintains a specific territory within the nucleus. This raises intriguing questions about where and when coinfecting viral genomes interact. To study the spatiotemporal requirements for intergenomic recombination, we developed an assay with dual-color FISH that enables detection of HR between different pairs of coinfecting HSV-1 genomes. Our results revealed... More