Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … The construct encoding TRIM25 with an N- terminal Flag epitope tag (DYKDDDDK) has been described previously35.The Flag-tagged human Riplet construct was obtained from GenScript in a pcDNA 3.1 expression vector. Constructs for … | Get A Quote |
RIG-I-MAVS antiviral signaling represents an important pathway to stimulate interferon production and confer innate immunity to the host. Upon binding to viral RNA and Riplet-mediated polyubiquitination, RIG-I promotes prion-like aggregation and activation of MAVS. MAVS subsequently induces interferon production by activating two signaling pathways mediated by TBK1-IRF3 and IKK-NF-κB respectively. However, the mechanism underlying the activation of MAVS downstream pathways remains elusive. Here, we demonstrated that activation of TBK1-IRF3 by MAVS-Region III depends on its multimerization state and identified TRAF3IP3 as a critical regulator for the downstream signaling. In response to virus infection,... More