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Custom Vector Construction> | … The genes of light and heavy chains were synthesized (Genscript, Piscataway, NJ), and cloned into a pCDNA3.1(+) vector with CD5 signal peptide, His 6 -tag, and tobacco etch virus (TEV) protease site sequences added at the N-terminus of the expression constructs … | Get A Quote |
Antigen recognition by mammalian antibodies represents the most diverse setting for protein-protein interactions, because antibody variable regions contain exceptionally diverse variable gene repertoires of DNA sequences containing combinatorial, non-templated junctional mutational diversity. Some animals use additional strategies to achieve structural complexity in the antibody combining site, and one of the most interesting of these is the formation of ultralong heavy chain complementarity determining region 3 loops in cattle. Repertoire sequencing studies of bovine antibody heavy chain variable sequences revealed that bovine antibodies can contain heavy chain complementarity determining region 3 (CDRH3... More