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DNA Sequencing> | … The synthetic gene asnB (UniprotKB – P06608) (sequence in Supplemental data 1) was purchased from GenScript, and further used as a template to be amplified in two different ways: adding the His-tag in the C-terminus (forward 5 … | Get A Quote |
L-Asparaginase (L-ASNase) is an important enzyme used to treat acute lymphoblastic leukemia, recombinantly produced in a prokaryotic expression system. Exploration of alternatives production systems like as extracellular expression in microorganisms generally recognized as safe (such as Glycoswitch) could be advantageous, in particular, if this system is able to produce homogeneous glycosylation. Here, we evaluated extracellular expression into Glycoswitch using two different strains constructions containing the gene coding for L-ASNase (with and without His-tag), in order to find the best system for producing the extracellular and biologically active protein. When the His-tag was absent, both ce... More