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Esterification of geraniol as a strategy for increasing product titre and specificity in engineered Escherichia coli.

Microb. Cell Fact.. 2019; 
ChacónMicaela G,MarriottAlice,KendrickEmanuele G,StylesMatthew Q,LeakDav
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Bacterial Expression System … ability to produce short chain fatty acids, overexpressed them in E. coli and analyzed role of host fatty acid synthesis pathway and its … glutamicum were codon optimized along with C-terminal 6xHis-tag, commercially synthesized and cloned in pUC57 vector by Genscript, USA to … Get A Quote

摘要

Geraniol, an acyclic monoterpene alcohol, is found as a primary constituent in the essential oils of plants such as geranium, lemongrass and rose. The floral-like scent of geraniol has made it a popular constituent of flavour and fragrance products. Over recent decades biotechnology has made significant progress towards the development of industrial platforms for the production of commercially valuable monoterpenoids, such as geraniol, through expression of recombinant terpene biosynthetic pathways in microbial hosts. Titres, however, have been hindered due to the inherent toxicity of these compounds-which are often utilised for anti-microbial and anti-fungal functions in their host plant.

关键词

Alcohol acyltransferase,Escherichia coli,Geraniol,Geranyl acetate,Microbial produc