Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | Both biotin-SDC4cyt and biotinpS179-SDC4cyt were recognized by anti-syndecan4cyt (custom made, with an internal epitope, GenScript) (Supporting Fig. S1A, upper panel), biotin-pS179-SDC4 was only recognized by antipS179-syndecan-4 (custom made, GenScript) (Supporting Fig. S1A, middle panel), whereas all three peptides were recognized by anti-biotin-HRP (Supporting Fig. S1A, lower panel). Peptides were synthesized to > 80% purity by GenScript. 12xHis-Ezrin (1-333) (rat, Uniprot P31977) was made by GenScript. Cloning was done by GenScript and all genes were tagged with either HA or FLAG tags in the pCEP4 or pcDNA3.1 vectors (GenScript). | Get A Quote |
Costameres are signaling hubs at the sarcolemma and important contact points between the extracellular matrix and cell interior, sensing and transducing biomechanical signals into a cellular response. The transmembrane proteoglycan syndecan-4 localizes to these attachment points and has been shown to be important in the initial stages of cardiac remodeling, but its mechanistic function in the heart remains insufficiently understood. Here, we sought to map the cardiac interactome of syndecan-4 to better understand its function and downstream signaling mechanisms. By combining two different affinity purification methods with MS analysis, we found that the cardiac syndecan-4 interactome consists of 21 nove... More