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Custom Vector Construction> | Genes of HIV-1 gp120 (residues 1-507) from the isolate 92BR020 with a C-terminal 6xhistidine tag, and of 4 domain CD4 (residues 1-388) with a C-terminal twin strep tag [(GGGGS)2WSHPQFEK(GGGGS)2WSHPQFEK)] were synthesized by GenScript (Piscataway, NJ) and cloned into pCMV-IRES-puro vector. | Get A Quote |
HIV-1 envelope glycoprotein (Env), which consists of trimeric (gp160) cleaved to (gp120 and gp41), interacts with the primary receptor CD4 and a coreceptor (such as chemokine receptor CCR5) to fuse viral and target-cell membranes. The gp120-coreceptor interaction has previously been proposed as the most crucial trigger for unleashing the fusogenic potential of gp41. Here we report a cryo-electron microscopy structure of a full-length gp120 in complex with soluble CD4 and unmodified human CCR5, at 3.9 Å resolution. The V3 loop of gp120 inserts into the chemokine-binding pocket formed by seven transmembrane helices of CCR5, and the N terminus of CCR5 contacts the CD4-induced bridging sheet of gp120. CCR... More