In the enzymatic conversion of biomass， it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc)， an essential member of cellulases， plays a critical role in cellulosic biomass degradation. The difficulty in improving the stability of Bgluc has been a bottleneck in the enzyme-dependent cellulose degradation. The traditional method of protein purification， however， leads to higher production cost and a decrease in activity. To simplify and efficiently purify Bgluc with modified special properties， Bgluc-tagged ELP and His with defined phase transitions was designed to facilitate the process.
In the enzymatic conversion of biomass， it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc)， an essential member of cellulases， plays a critical role in cellulosic biomass degradation. The difficulty in improving the stability of Bgluc has been a bottleneck in the enzyme-dependent cellulose degradation. The traditional method of protein purification， however， leads to higher production cost and a decrease in activity. To simplify and efficiently purify Bgluc with modified special properties， Bgluc-tagged ELP and His with defined phase transitions was designed to facilitate the process.
