Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | Plasmids used for transfection include pcDNA3.1-Flag-STN1 pcDNA3.1-HA-CTC1 pTRE2-TEN1, pInducer20-Blast-CDT1-HA , and pcDNA3.1 Flag-tagged MCM subunits (ORF cDNA clones from GenScript). | Get A Quote |
Human CTC1-STN1-TEN1 (CST) is an RPA-like single-stranded DNA-binding protein that interacts with DNA polymerase α-primase (pol α) and functions in telomere replication. Previous studies suggest that CST also promotes replication restart after fork stalling. However, the precise role of CST in genome-wide replication remains unclear. In this study, we sought to understand whether CST alters origin licensing and activation. Replication origins are licensed by loading of the minichromosome maintenance 2-7 (MCM) complex in G1 followed by replisome assembly and origin firing in S-phase. We find that CST directly interacts with the MCM complex and disrupts binding of CDT1 to MCM, leading to decreased origin ... More