Products/Services Used | Details | Operation |
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Gene Synthesis> | Stub1 was purchased from Harvard Medical school, pUC57 Foxp3 7xA and 7xD were synthesized by GenScript Biotech, and cloned into pMT2 containing an N-terminal HA-tag generating pMT2-HA-Stub1, pMT2-HA-Foxp3 7xA and 7xD respectively. | Get A Quote |
The Foxp3 transcription factor is a crucial determinant of both regulatory T (T) cell development and their functional maintenance. Appropriate modulation of tolerogenic immune responses therefore requires the tight regulation of Foxp3 transcriptional output, and this involves both transcriptional and post-translational regulation. Here, we show that during T cell activation, phosphorylation of Foxp3 in T cells can be regulated by a TGF-β activated kinase 1 (TAK1)-Nemo-like kinase (NLK) signaling pathway. NLK interacts and phosphorylates Foxp3 in T cells, resulting in the stabilization of protein levels by preventing association with the STUB1 E3-ubiquitin protein ligase. Conditional T cell NLK-knocko... More