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Enhanced oxygen consumption in Herbaspirillum seropedicae fnr mutants leads to increased NifA mediated transcriptional activation.

BMC Microbiol.. 2015;

BatistaMarcelo Bueno，WassemRoseli，PedrosaFábio de Oliveira，de SouzaEmanuel Maltempi，DixonRay，MonteiroRose A

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	To construct the C-terminal 3xFlag tagged NifA strains, we generated a nifA-3XFlag gene by cloning the complete nifA gene (1629 bp) in frame with the 3xFlag sequence from a vector synthesized by the GenScript® Corporation (Table 1).	Get A Quote

摘要

Orthologous proteins of the Crp/Fnr family have been previously implicated in controlling expression and/or activity of the NifA transcriptional activator in some diazotrophs. This study aimed to address the role of three Fnr-like proteins from H. seropedicae SmR1 in controlling NifA activity and consequent NifA-mediated transcription activation.