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Proteins, Expression, Isolation and Analysis> | The eGFP-CEN2 CDS with a Kozak sequence and flanking PmeI and RsrII sites was synthesized (GenScript Inc, NJ) and ligated via the PmeI and RsrII sites. Journal of Cell Science • Accepted manuscript Page 14. pmin … | Get A Quote |
Centrins are EF-hand containing proteins ubiquitously found in eukaryotes and are key components of centriolesasal bodies as well as certain contractile fibers. We previously identified three centrins in the human parasite , all of which localized to the centrioles. However, one of them, (Tg) Centrin2 (CEN2), is also targeted to structures at the apical and basal ends of the parasite, as well as to annuli at the base of the apical cap of the membrane cortex. The role(s) that CEN2 play in these locations were unknown. Here, we report the functional characterization of CEN2 using a conditional knockdown method that combines transcriptional and protein stability control. The knockdown resulted in an o... More