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Custom Vector Construction> | The cDNA coding for the ADAM17-specific scFv (A300EscFv) was synthesized by GenScript and then subcloned into the bacterial expression vector pET23a (Novagen) which contained a pelB-based leader sequence upstream and sequences coding for the c-Myc and His tags downstream of the scFv cDNA insert. | Get A Quote |
ADAM17 (A disintegrin and metalloproteinase 17) is a membrane-bound protease that cleaves various cell surface proteins, including cytokines and cytokine receptors. Recently it was shown that ADAM17 is highly expressed on the surface of many cancer cells, whereas normal cells express low levels of ADAM17, implying that ADAM17 is a potential immunotherapeutic target. We have generated a monoclonal antibody against human ADAM17, which recognized the membrane proximal cysteine-rich extension of the ADAM17 protein. Unlike normal cells, tumour cell lines, such as a prostate cancer cell line, pancreatic cancer cell lines, a breast cancer cell line and a non-small lung cancer cell line, expressed ADA... More