Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | … The blots were incubated with specific primary rabbit anti-His-tag polyclonal (GenScript, USA), followed by HRP-conjugated secondary antibody, and detected by an enhanced chemiluminescent substrate. 3. Results and discussion. 3.1. The cloning of aroGM150 gene … | Get A Quote |
3-Deoxy-d-arabino-heptulonate-7-phosphate (DAHP) synthase is one of the key enzymes, which catalyzes the first step in the aromatic amino acid biosynthetic pathway and yields the three amino acids tyrosine (Tyr), tryptophan (Typ), and phenylalanine (Phe). In Escherichia coli (E. coli), three differently regulated DAHP synthases carry out the first regulated step in the aromatic amino acid biosynthetic pathway. The three DAHP synthases encoded by the genes aroG, aroF, and aroH are inhibited by phenylalanine, tyrosine and tryptophan, respectively. In this work, the aroG gene was cloned and mutated by site-directed mutagenesis using overlap extension PCR (SOE-PCR) technique. The feedback-resistan... More