A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction， ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column). The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography， monosaccharide composition， Fourier transform infrared spectrometry， UV-visible spectrophotometry， scanning electron microscope and Congo red test. The results indicated that DIP-1 was consisted of mannose， glucosamine， glucose， galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81， and its molecular weight was 218.3 kDa. Meanwhile， DIP-1 presented a straight hex... More
A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction， ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column). The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography， monosaccharide composition， Fourier transform infrared spectrometry， UV-visible spectrophotometry， scanning electron microscope and Congo red test. The results indicated that DIP-1 was consisted of mannose， glucosamine， glucose， galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81， and its molecular weight was 218.3 kDa. Meanwhile， DIP-1 presented a straight hexahedron structure， but no triple-helical conformation. In antioxidant activity tests， DIP-1 exhibited powerful scavenging activities on hydroxyl， DPPH， ABTS radicals and reducing power in a dose-dependent manner. Especially， DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro， with IC values of 1.42 and 1.23 mg/ml， respectively. PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases， including cancer， inflammation， leprosy， fever， bleeding and so on. At present， polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties， including antitumor， antidiabetic， antioxidant and immunomodulatory activity. In the present study， we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover， the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore， the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.
