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Gene Synthesis> | … The TrCBM1 is a family 1 CBM taken from the CBH I gene of T. reesei. It was synthesized (GenScript Company, Piscataway, NJ) with genetic code optimization for expression in E. coli. The gene also includes a sequence encoding an N-terminal 6xHis tag … | Get A Quote |
LPMO (lytic polysaccharide monooxygenase) represents a unique paradigm of cellulosic biomass degradation by an oxidative mechanism. Understanding the role of LPMO in deconstructing crystalline cellulose is fundamental to the enzyme's biological function and will help to specify the use of LPMO in biorefinery applications. Here we show with real-time atomic force microscopy that C1 and C4 oxidizing types of LPMO from Neurospora crassa (NcLPMO9F, NcLPMO9C) bind to nanocrystalline cellulose with high preference for the very same substrate surfaces that are also used by a processive cellulase (Trichoderma reesei CBH I) to move along during hydrolytic cellulose degradation. The bound LPMOs, however, are immobi... More