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Plasmid DNA Preparation> | … tag, was a gift from Dr. Bernhard Lagger- bauer.56 A phosphorylated mimic of the FMRP ISO1, FMRP S500D, was designed by replacing serine 500 with aspartic acid to mimic phosphorylation.50 The pET-21a-FMRP FMRP S500D plasmid was produced by GenScript USA … | Get A Quote |
Fragile X syndrome (FXS), the most common cause of inherited intellectual disability, is caused by the loss of expression of the fragile X mental retardation protein (FMRP). FMRP, which regulates the transport and translation of specific mRNAs, uses its RGG box domain to bind mRNA targets that form G-quadruplex structures. One of the FMRP in vivo targets, Shank1 mRNA, encodes the master scaffold proteins of the postsynaptic density (PSD) which regulate the size and shape of dendritic spines because of their capacity to interact with many different PSD components. Due to their effect on spine morphology, altered translational regulation of Shank1 transcripts may contribute to the FXS pathology. We ... More