Products/Services Used | Details | Operation |
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Custom Vector Construction> | … The plasmid vector can be purchased from one of biotech companies such as Promega (Cat. Nos. C8750, C8760, C8770, C8780, C8790, C7800, etc) and Genscript (Cat. Nos. SD1201, SD1202, SD1207, etc), or can be constructed … | Get A Quote |
Bacterial DNA contains CpG oligonucleotide (ODN) motifs to trigger innate immune responses through the endosomal receptor Toll-like receptor 9 (TLR9). One of the cell surface receptor to capture and deliver microbial DNA to intracellular TLR9 is the C-type lectin molecule DEC-205 through its N-terminal C-type lectin like domain (CTLD). CD93 is a cell surface protein and member of the lectin group XIV with a CTLD. We hypothesized that CD93 could interact with CpG motifs, and possibly serving as a novel receptor to deliver bacterial DNA to endosomal TLR9. Using ELISA and tryptophan fluorescence binding studies we observed that the soluble histidine-tagged CD93-CTLD was specifically binding to CpG ODN and bacter... More