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Monoclonal Antibody Services> | … The BCA protein assay reagent kit was pur- chased from Pierce. Mouse Anti-His-tag Monoclonal Antibody and HRP-Goat anti-mouse IgG (H+L) were purchased from GenScript USA Inc. (Nanjing, China). Coomassie Brilliant Blue G-250 was from Fluka … | Get A Quote |
Nattokinase (NK) is a serine protease of the subtilisin family; as a potent fibrinolytic enzyme, it is potentially useful for thrombosis therapy. For NK to be applied as an oral medicine for the treatment of cardiovascular diseases, it must overcome the extremely acidic environments of the gastrointestinal tract despite its limited acidic stability. In this study, three strategies were adopted to improve the acid resistance of NK: (a) Surface charge engineering, (b) sequence alignment, and (c) mutation based on the literature. Eleven variants were constructed and four single-point mutations were screened out for their distinctive catalytic properties: Q59E increased the specific activity; S78T improv... More