| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | All subsequent steps were performed in DNA LoBind tubes (Eppendorf, 22431021) and each step was performed in a fresh tube. Then, 100 μl of streptavidin Dynabeads (MyOne Streptavin C1 Beads, Invitrogen, 650-01) were washed twice with 400 μl Tween wash buffer (5 mM Tris-HCl pH 8.0, 0.5 mM EDTA, 1 M NaCl, 0.05% Tween-20) by incubating for 3 min at room temperature with rotation, reclaiming against a magnetic separation rack (Genscript, M00140) for 1 min and removing all supernatant. | Get A Quote |
