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Custom Vector Construction> | The Burkholderia diffusa gene encoding for ObiF1 (ObiF1; KUZ09184.1) was synthesized, codon optimized (Supplementary Fig. 12), and cloned into a pET28 vector (NdeI/HindIII) encoding for an N-terminal hexahistidine tag (pET28-ObiF1; Genscript; Piscataway, NJ, USA). I | Get A Quote |
Nonribosomal peptide synthetases produce diverse natural products using a multidomain architecture where the growing peptide, attached to an integrated carrier domain, is delivered to neighboring catalytic domains for bond formation and modification. Investigation of these systems can lead to the discovery of new structures, unusual biosynthetic transformations, and to the engineering of catalysts for generating new products. The antimicrobial β-lactone obafluorin is produced nonribosomally from dihydroxybenzoic acid and a β-hydroxy amino acid that cyclizes into the β-lactone during product release. Here we report the structure of the nonribosomal peptide synthetase ObiF1, highlighting the structur... More