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Proteins, Expression, Isolation and Analysis> | … total immunoglobulin. The collected immunoglobulin was used for IgG purification. The IgG was collected by using protein G columns (GenScript, Nanjing, China) according to the manufacturer's instructions. The concentration … | Get A Quote |
To investigate the potential role of β-galactosidase in altering immunoglobulin G (IgG) galactosylation in serum of rheumatoid arthritis (RA). The expression level and activity of β-galactosidase in serum and CD 19 B cells were measured by enzyme-linked immune sorbent assay (ELISA). The effect of β-galactosidase on the -glycan changes in serum from mice intravenously treated with β-galactosidase was observed by linear ion-trap quadrupole-electrospray ionization mass spectrometry (LTQ-ESI-MS). We established a collagen-induced arthritis (CIA) rat model to explore the biological function of β-galactosidase in RA. The expression level of β-galactosidase in serum of 32 patients was elevated when compared wi... More