| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | Nucleotide sequences encoding PR10 and MLP candidates and domain-swap mutants were codon optimized for expression in Escherichia coli and synthesized (GenScript) with the addition of an N-terminal His6 tag for insertion into pACE or pET19b vectors. | Get A Quote |
The isomerization of neopinone to codeinone is a critical step in the biosynthesis of opiate alkaloids in opium poppy. Previously assumed to be spontaneous， the process is in fact catalyzed enzymatically by neopinone isomerase (NISO). Without NISO the primary metabolic products in the plant， in engineered microbes and in vitro are neopine and neomorphine， which are structural isomers of codeine and morphine， respectively. Inclusion of NISO in yeast strains engineered to convert thebaine to natural or semisynthetic opiates dramatically enhances formation of the desired products at the expense of neopine and neomorphine accumulation. Along with thebaine synthase， NISO is the second member of the pathoge... More
