Products/Services Used | Details | Operation |
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Bacterial Expression System> | .... isolation and purification The recombinant LngA-His protein was prepared by GenScript (Piscataway, NJ.... and immunoblotting LngA-His recombinant protein was prepared by GenScript (Piscataway, NJ) and CS21 and CS8 pili.... ETEC strain; LngA-his tag protein was obtained from Genscript; BL21 E. coli was used as negative | Get A Quote |
CS21 pili of enterotoxigenic Escherichia coli (ETEC) is one of the most prevalent ETEC colonization factors. CS21 major subunit, LngA, mediates ETEC adherence to intestinal cells, and contributes to ETEC pathogenesis in a neonatal mouse infection model. The objectives of this work were to evaluate LngA major subunit purified protein and CS21 purified pili on immunogenicity and protection against ETEC colonization of mice intestine. Recombinant LngA purified protein or purified CS21 pili from E9034A ETEC strain were evaluated for immunogenicity after immunization of C57BL/6 mice. Specific anti-LngA antibodies were detected from mice serum, feces, and intestine fluid samples by ELISA assays. Protection ... More